The pichia pastoris recombinant strain was induced by methanol addition to the culture medium Sds - page分析表明,在63kd處有明顯的蛋白質(zhì)特征帶。
Expression of cyt1aa from bacillus thuringiensis in bacillus thuringiensis var . tenebrionis strain and the insecticidal activity of the recombinant strain 基因在擬步甲亞種菌株中的表達(dá)及重組菌株殺蟲活性研究
The gene recombinant strain no . 42 ca n ' t generate ampramycin , which indicated that the cloned gene is involved in apramycin biosynthesis in s . tenebrarius 通過接合轉(zhuǎn)移的方法將質(zhì)粒pzxb014導(dǎo)入黑暗鏈霉菌h6中,篩選基因發(fā)生重組的菌株。
Finally , the growth conditions , growth curves , fermentation conditions of recombinant strains and the biological properties of purified recombinant protein were investigated 最后對(duì)重組菌的生長(zhǎng)條件、生長(zhǎng)曲線、發(fā)酵條件和表達(dá)產(chǎn)物的體外生物學(xué)活性進(jìn)行了研究。
Some important factors of high density fermentation of genetic engineering microorganisms including the construct of recombinant strains , culture conditions , growth inhibitor and the process control were described 摘要闡述了基因工程菌高密度發(fā)酵工藝的幾個(gè)主要影響因素,包括重組菌構(gòu)建、培養(yǎng)條件、生長(zhǎng)抑制因數(shù)以及它們的控制技術(shù)。
Object : the culture medium and culture conditions of psb will be optimized and gene ubia will be cloned for the construction of recombinant strain producing and foundation of the fermentative technology in large scale 目的:優(yōu)化psb產(chǎn)coq _ ( 10 )培養(yǎng)基及培養(yǎng)條件和克隆ubia基因,為獲取高產(chǎn)coq _ ( 10 )基因工程菌及確定規(guī)?;l(fā)酵工藝奠定良好的基礎(chǔ)。
The three recombinant strains were induced to express with iptg . the tannase activity was detected by ultravillet spectroscopy and the target protein was found in sds - page with the correct size of 63kd . the gene was also insered in the plasmid ppic9k of pichia pastoris 將單寧酶基因tan重組到trc啟動(dòng)子控制下的表達(dá)載體pse380中,并分別轉(zhuǎn)化入大腸桿菌dh5 , top10 , bl21 ( de3 )三種重組表達(dá)菌株中。
One highly productive and genetically stable recombinant strain named e - 22 , which produced phytase with 143958 . 3u / ml under the condition of flask cultivation , was selected through further screening . the phytase activity of e - 22 was 341 . 13 times as high as that of the original strain ( 422u / ml ) 經(jīng)搖瓶復(fù)篩后得到l株產(chǎn)酶活性為143958 . 3uzml發(fā)酵液,并且具有良好遺傳穩(wěn)定性的高產(chǎn)工程菌株( e22 ) ,其產(chǎn)酶活性是出發(fā)菌株酶活性( 422u / ml )的341
The genes encoding pyruvate decarboxylase ( pdc ) and alcohol dehydrogenase ii ( adh ii ) were amplified from total dna of zymomonas mobilis by pcr . the genes of adhb andpdc were inserted into expression vector pse380 and then transformed into e . coli dh5a . the recombinant strains were induced to express adh ii and pdc with iptg 本論文以zymomonasmobilisdna為模板, pcr擴(kuò)增zymomonasmobilis中的乙醇脫氫酶基因( adhb )和丙酮酸脫羧酶基因( pdc ) ,分別構(gòu)建表達(dá)質(zhì)粒pse - adhb和pse - pdc并在大腸桿菌dh5中表達(dá)。
It was confirmed that the key enzyme of coq10 synthesis is p - hydroxybenzoate polyprenyltransferase , encoded by gene ubia in e . coli and lacks specificity to the aggregation length of its substrate ( polyisoprenyl pyrophosphate ) . if gene ubia can be introduced into psb and is high expression in psb , a recombinant strain producing coq10 could be obtained . consequently , the productive cost will decrease sharply 業(yè)已證實(shí)coq _ ( 10 )生物合成的關(guān)鍵酶為對(duì)羥基苯甲酸聚異戊二烯焦磷酸轉(zhuǎn)移酶(在大腸桿菌中該酶由ubia基因編碼) ,該類酶對(duì)底物聚異戊二烯焦磷酸( ppp )的聚合長(zhǎng)度并無特殊要求。